ERDDAP > info > CruiseBacteria

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
	set	data	graph		files	Bacterial properties in discrete water column samples at selected depths, collected aboard Palmer LTER annual cruises off the coast of the Western Antarctica Peninsula, 2003, 2019.		F   I   M	background			National Science ...	CruiseBacteria

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	_NCProperties	String	version=1|netcdflibversion=4.6.1|hdf5libversion=1.10.6
attribute	NC_GLOBAL	acknowledgement	String	Funding and support provided by the National Science Foundation
attribute	NC_GLOBAL	cdm_data_type	String	Trajectory
attribute	NC_GLOBAL	cdm_trajectory_variables	String	study_name
attribute	NC_GLOBAL	comment	String	The Palmer, Antarctica, Long-Term Ecological Research project is a member site of the Long-Term Ecological Research program, a network of sites investigating diverse biomes. A team of researchers seeks to understand the structure and function of the Western Antarctic Peninsula's marine and terrestrial ecosystems in the context of seasonal-to-interannual atmospheric and sea ice dynamics, as well as long-term climate change. The PAL measurement system (or grid) is designed to study marine and terrestrial food webs consisting principally of diatom primary producers, the dominant herbivore Antarctic krill, and the apex predator Adelie penguin. An attenuated microbial food web is also a focus. PAL studies these ecosystems annually over a regional scale grid of oceanographic stations and seasonally at Palmer Station. \n\nPalmer Station is located on Anvers Island west of the Antarctic Peninula. The peninsula runs perpendicular to a strong climatic gradient between the cold, dry continental regime to the south, characteristic of the Antarctic interior, and the warm, moist, maritime regime to the north. North-south shifts in the gradient give rise to large environmental variability to climate change. Sea ice extent and variability affects ecosystem changes at all trophic levels. In addition to the long-term field and research activities, information management, graduate student training, education and outreach are an integral part of the program.
attribute	NC_GLOBAL	contributor_email	String	hducklow at ldeo.columbia.edu
attribute	NC_GLOBAL	contributor_name	String	Hugh Ducklow
attribute	NC_GLOBAL	contributor_role	String	PrincipalInvestigator
attribute	NC_GLOBAL	contributor_role_vocabulary	String	https://vocab.nerc.ac.uk/collection/G04/current/
attribute	NC_GLOBAL	Conventions	String	CF-1.8, ACDD-1.3, COARDS
attribute	NC_GLOBAL	creator_country	String	USA
attribute	NC_GLOBAL	creator_email	String	kerfoot at marine.rutgers.edu
attribute	NC_GLOBAL	creator_institution	String	Rutgers University
attribute	NC_GLOBAL	creator_name	String	John Kerfoot
attribute	NC_GLOBAL	creator_sector	String	academic
attribute	NC_GLOBAL	creator_type	String	person
attribute	NC_GLOBAL	creator_url	String	https://rucool.marine.rutgers.edu
attribute	NC_GLOBAL	datazoo_dataset_id	String	48
attribute	NC_GLOBAL	datazoo_datatable_id	String	48
attribute	NC_GLOBAL	datazoo_datatable_label	String	Bacteria (Cruise)
attribute	NC_GLOBAL	datazoo_datatable_name	String	Bacteria
attribute	NC_GLOBAL	date_created	String	2021-05-07T15:54:36Z
attribute	NC_GLOBAL	date_issued	String	2021-05-07T15:54:36Z
attribute	NC_GLOBAL	defaultDataQuery	String	null
attribute	NC_GLOBAL	defaultGraphQuery	String	longitude,latitude,time&.draw=markers&.marker=6%7C5&.color=0x000000&.colorBar=%7C%7C%7C%7C%7C&.bgColor=0xffccccff
attribute	NC_GLOBAL	doi	String	https://doi.org/10.6073/pasta/32683b1152c89e8eb8879639146b34fe
attribute	NC_GLOBAL	Easternmost_Easting	double	-64.02944
attribute	NC_GLOBAL	featureType	String	Trajectory
attribute	NC_GLOBAL	geospatial_bounds_crs	String	EPSG:4326
attribute	NC_GLOBAL	geospatial_bounds_vertical_crs	String	EPSG:5831
attribute	NC_GLOBAL	geospatial_lat_max	double	-63.59
attribute	NC_GLOBAL	geospatial_lat_min	double	-70.088
attribute	NC_GLOBAL	geospatial_lat_resolution	String	0.00001 degree
attribute	NC_GLOBAL	geospatial_lat_units	String	degrees_north
attribute	NC_GLOBAL	geospatial_lon_max	double	-64.02944
attribute	NC_GLOBAL	geospatial_lon_min	double	-78.208
attribute	NC_GLOBAL	geospatial_lon_resolution	String	0.00001 degree
attribute	NC_GLOBAL	geospatial_lon_units	String	degrees_east
attribute	NC_GLOBAL	geospatial_vertical_max	double	5000.0
attribute	NC_GLOBAL	geospatial_vertical_min	double	-999.0
attribute	NC_GLOBAL	geospatial_vertical_positive	String	down
attribute	NC_GLOBAL	geospatial_vertical_units	String	m
attribute	NC_GLOBAL	history	String	local files
attribute	NC_GLOBAL	infoUrl	String	https://pal.lternet.edu/
attribute	NC_GLOBAL	institution	String	National Science Foundation
attribute	NC_GLOBAL	keywords	String	amino acids, bacteria, bacterial abundance, leucine, oceans, Organic Matter, Population Studies
attribute	NC_GLOBAL	keywords_vocabulary	String	LTER Controlled Vocabulary,LTER Core Areas
attribute	NC_GLOBAL	license	String	All data contained herein is licensed under the LTER Network Data Access Policy (https://lternet.edu/data-access-policy/). In accordance with scientific publishing conventions and professional etiquette, the consumer of these data (the "Data User") is expected to appropriately cite these data in any publication that results from their use. The Data User should realize that these data may be actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of these data if any questions about methodology or results occur and should realize that misinterpretation of data may occur if used out of context of the original study. Where appropriate, the Data User is encouraged to consider collaboration or co-authorship with the data authors. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed. All data are made available "as is." The Data User should be aware that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data.
attribute	NC_GLOBAL	license_link	String	https://lternet.edu/data-access-policy/
attribute	NC_GLOBAL	methods	String	General Methods: Water samples for all microbial analyses are harvested from the CTD-Rosette system. Bacterial abundance samples are analyzed within two hours by flow cytometry following the protocol of Gasol and del Giorgio (2000), with SYBR-Green staining on an Accuri C6 (Becton-Dickinson). Total bacteria concentrations were determined by adding 1 μm microspheres (Polysciences, Warrington, PA) and 5 μM final concentration of stain to 0.5 mL of sample. Samples were incubated in the dark for 30 minutes and analyzed for 2 minutes at a low flow rate. Numbers of total bacteria and beads were determined in cytograms of side scatter (SSC) versus green fluorescence (FL1). Calculation of the absolute concentration of stained particles was based on the total sample volume analyzed, as determined by the count of added microspheres.\n\n<em>Bacterial Production</em>\nBacterial Production (BP) rates were derived from rates of 3H-leucine incorporation measured on samples extending over the upper 50-100 m. The leucine assays followed a procedure modified from the protocol originally proposed by Smith and Azam (1992). Briefly, triplicate 1.5 ml samples were incubated shortly after collection for ~3 h with 3H-leucine (MP Biomedical, Santa Ana, CA; >100 Ci/mmol, 20-25 nM final concentration) in 2.0 ml microcentrifuge tubes (Axygen SCT-200, Union City, CA). Incubations were maintained within 0.5°C of the in situ temperature in refrigerated circulator baths and terminated by the addition of 0.1 ml of 100% trichloroacetic acid (TCA). Samples were concentrated by centrifugation, rinsed with 5% TCA and 70% ethanol and air-dried overnight prior to radioassay by liquid scintillation counting in Ultima Gold cocktail (Perkin-Elmer, Waltham, MA). Blank values of TCA-killed samples were subtracted from the average of the triplicates for each discrete depth sample. BP rates are derived by multiplying the leucine incorporation rate times 1500 gC mole-1 (Ducklow et al (2000).\n\n
attribute	NC_GLOBAL	naming_authority	String	edu.rutgers.rucool
attribute	NC_GLOBAL	Northernmost_Northing	double	-63.59
attribute	NC_GLOBAL	product_version	String	1.0
attribute	NC_GLOBAL	program	String	LTER
attribute	NC_GLOBAL	project	String	Palmer LTER
attribute	NC_GLOBAL	publisher_country	String	USA
attribute	NC_GLOBAL	publisher_email	String	kerfoot at marine.rutgers.edu
attribute	NC_GLOBAL	publisher_institution	String	Rutgers University
attribute	NC_GLOBAL	publisher_name	String	John Kerfoot
attribute	NC_GLOBAL	publisher_type	String	person
attribute	NC_GLOBAL	publisher_url	String	https://rucool.marine.rutgers.edu
attribute	NC_GLOBAL	references	String	https://pal.lternet.edu/,Gasol JM, Del Giorgio PA. 2000. Using flow cytometry for counting natural planktonic bacteria and understanding the structure of planktonic bacterial communities. SCIENTIA MARINA 64: 197-224.,Smith DC, Azam F. 1992. A simple, economical method for measuring bacterial protein synthesis rates in seawater using 3H-leucine. Marine Microbial Foodwebs 6: 107-114.,Ducklow HW, Dickson ML, Kirchman DL, Steward G, Orchardo J, Marra J, Azam F. 2000. Constraining bacterial production, conversion efficiency and respiration in the Ross Sea, Antarctica, January-February, 1997. Deep-Sea Research II 47: 3227-3247.\n
attribute	NC_GLOBAL	sea_name	String	Southern Ocean
attribute	NC_GLOBAL	source	String	local files
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	Southernmost_Northing	double	-70.088
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v77
attribute	NC_GLOBAL	subsetVariables	String	study_name,station
attribute	NC_GLOBAL	summary	String	Bacterial properties in discrete water column samples at selected depths, collected aboard Palmer LTER annual cruises off the coast of the Western Antarctica Peninsula, 2003 - 2019. The microbial biogeochemistry component of PAL focuses on marine bacterioplankton, and is thus a counterpart to the phytoplankton and zooplankton components, which together provide a detailed and comprehensive description of plankton ecology in PAL-LTER. Bacteria and Archaea (hereafter called "bacteria") are taxonomically and metabolically diverse. In coastal and offshore surface waters Bacteria generally predominate over Archaea, but Archaea are equal or greater in abundance in the mesopelagic layer below the euphoric zone. We focus on aerobic, heterotrophic bacteria in the upper 100 m on the annual summer cruise. These bacteria oxidize recently-produced low molecular weight dissolved organic compounds released by phytoplankton and zooplankton, decomposing them back into CO2 and inorganic nutrients. Globally, marine bacteria respire an amount of carbon roughly equal to about half the daily photosynthetic production. In cold polar waters, relative bacterial activity is lower, with bacterial biomass production being equal to <5% of the daily photosynthesis. The ratio at lower latitudes is 10-20%. The factors responsible for this contrast are not entirely clear. Resolving this pattern is a key aim of the PAL microbial component. Bacterial production is generally low across the grid, relative to primary production, but with considerable spatial and annual variability. Discrete BP can reach >200mgC/m2/d following bloom-fueled high organic matter events. Across the grid and over years, BP is highly correlated with chlorophyll, highlighting the close relationship with phytoplanktonic organic matter production.
attribute	NC_GLOBAL	time_coverage_end	String	2019-02-04T00:00:00Z
attribute	NC_GLOBAL	time_coverage_start	String	2003-01-06T01:35:52Z
attribute	NC_GLOBAL	title	String	Bacterial properties in discrete water column samples at selected depths, collected aboard Palmer LTER annual cruises off the coast of the Western Antarctica Peninsula, 2003, 2019.
attribute	NC_GLOBAL	Westernmost_Easting	double	-78.208
variable	study_name		String
attribute	study_name	cf_role	String	trajectory_id
attribute	study_name	column_name	String	studyName
attribute	study_name	comment	String	Cruise name
attribute	study_name	ioos_category	String	Identifier
attribute	study_name	long_name	String	Study
variable	event		short
attribute	event	_FillValue	short	32767
attribute	event	actual_range	short	6, 790
attribute	event	colorBarMaximum	double	100.0
attribute	event	colorBarMinimum	double	0.0
attribute	event	column_name	String	col_588
attribute	event	comment	String	Event number from event log
attribute	event	ioos_category	String	Statistics
attribute	event	long_name	String	Event Number
attribute	event	lter_mscale	String	interval
attribute	event	lter_num_type	String	real
attribute	event	lter_units_string	String	number
attribute	event	units_conventions	String	EML-2.0.1,EML-2.1.0,EML-LTER
variable	station		String
attribute	station	column_name	String	col_589
attribute	station	comment	String	Palmer LTER grid station
attribute	station	ioos_category	String	Identifier
attribute	station	long_name	String	Station Name
attribute	station	lter_mscale	String	nominal
variable	bottle		byte
attribute	bottle	_FillValue	byte	127
attribute	bottle	actual_range	byte	0, 25
attribute	bottle	colorBarMaximum	double	100.0
attribute	bottle	colorBarMinimum	double	0.0
attribute	bottle	column_name	String	col_590
attribute	bottle	comment	String	Reference number of sample bottle from rosette
attribute	bottle	ioos_category	String	Statistics
attribute	bottle	long_name	String	Bottle Number
attribute	bottle	lter_mscale	String	interval
attribute	bottle	lter_num_type	String	natural
attribute	bottle	lter_units_string	String	number
attribute	bottle	units_conventions	String	EML-2.0.1,EML-2.1.0,EML-LTER
variable	time		double
attribute	time	_CoordinateAxisType	String	Time
attribute	time	actual_range	double	1.041816952E9, 1.5492384E9
attribute	time	axis	String	T
attribute	time	calendar	String	proleptic_gregorian
attribute	time	column_name	String	col_591
attribute	time	comment	String	Datetime GMT from CTD bottle trip record
attribute	time	ioos_category	String	Time
attribute	time	long_name	String	Datetime GMT
attribute	time	lter_mscale	String	dateTime
attribute	time	standard_name	String	time
attribute	time	time_origin	String	01-JAN-1970 00:00:00
attribute	time	units	String	seconds since 1970-01-01T00:00:00Z
variable	latitude		double
attribute	latitude	_CoordinateAxisType	String	Lat
attribute	latitude	_FillValue	double	9.969209968386869E36
attribute	latitude	actual_range	double	-70.088, -63.59
attribute	latitude	axis	String	Y
attribute	latitude	colorBarMaximum	double	90.0
attribute	latitude	colorBarMinimum	double	-90.0
attribute	latitude	column_name	String	col_4476
attribute	latitude	comment	String	Latitude from CTD bottle trip record
attribute	latitude	ioos_category	String	Location
attribute	latitude	long_name	String	Latitude
attribute	latitude	lter_mscale	String	ratio
attribute	latitude	lter_num_type	String	real
attribute	latitude	lter_units	String	º
attribute	latitude	lter_units_string	String	degree
attribute	latitude	standard_name	String	latitude
attribute	latitude	units	String	degrees_north
attribute	latitude	units_conventions	String	EML-2.0.1,EML-2.1.0,EML-LTER
variable	longitude		double
attribute	longitude	_CoordinateAxisType	String	Lon
attribute	longitude	_FillValue	double	9.969209968386869E36
attribute	longitude	actual_range	double	-78.208, -64.02944
attribute	longitude	axis	String	X
attribute	longitude	colorBarMaximum	double	180.0
attribute	longitude	colorBarMinimum	double	-180.0
attribute	longitude	column_name	String	col_4477
attribute	longitude	comment	String	Longitude from CTD bottle trip record
attribute	longitude	ioos_category	String	Location
attribute	longitude	long_name	String	Longitude
attribute	longitude	lter_mscale	String	ratio
attribute	longitude	lter_num_type	String	real
attribute	longitude	lter_units	String	º
attribute	longitude	lter_units_string	String	degree
attribute	longitude	standard_name	String	longitude
attribute	longitude	units	String	degrees_east
attribute	longitude	units_conventions	String	EML-2.0.1,EML-2.1.0,EML-LTER
variable	depth		double
attribute	depth	_CoordinateAxisType	String	Height
attribute	depth	_CoordinateZisPositive	String	down
attribute	depth	_FillValue	double	9.969209968386869E36
attribute	depth	actual_range	double	-999.0, 5000.0
attribute	depth	axis	String	Z
attribute	depth	colorBarMaximum	double	8000.0
attribute	depth	colorBarMinimum	double	-8000.0
attribute	depth	colorBarPalette	String	TopographyDepth
attribute	depth	column_name	String	col_592
attribute	depth	comment	String	Target depth for sample. Not actual depth from instrument record
attribute	depth	ioos_category	String	Location
attribute	depth	long_name	String	Depth
attribute	depth	lter_mscale	String	ratio
attribute	depth	lter_num_type	String	real
attribute	depth	lter_units	String	m
attribute	depth	lter_units_string	String	meter
attribute	depth	positive	String	down
attribute	depth	standard_name	String	depth
attribute	depth	units	String	m
attribute	depth	units_conventions	String	AND-LTER,BNZ-LTER,CDR-LTER,EML-2.0.1,EML-2.1.0,EML-LTER,LUQ-LTER,SGS-LTER
variable	bacterial_cell_count		double
attribute	bacterial_cell_count	_FillValue	double	9.969209968386869E36
attribute	bacterial_cell_count	actual_range	double	-999.0, 4.9E9
attribute	bacterial_cell_count	colorBarMaximum	double	100.0
attribute	bacterial_cell_count	colorBarMinimum	double	0.0
attribute	bacterial_cell_count	column_name	String	col_593
attribute	bacterial_cell_count	comment	String	Bacterial abundance
attribute	bacterial_cell_count	ioos_category	String	Statistics
attribute	bacterial_cell_count	long_name	String	Abundance
attribute	bacterial_cell_count	lter_mscale	String	ratio
attribute	bacterial_cell_count	lter_num_type	String	real
attribute	bacterial_cell_count	lter_units	String	num/L
attribute	bacterial_cell_count	lter_units_string	String	numberPerLiter
attribute	bacterial_cell_count	units	String	count L-1
attribute	bacterial_cell_count	units_conventions	String	EML-2.0.1,EML-2.1.0,EML-LTER,NTL-LTER
variable	thymidine		double
attribute	thymidine	_FillValue	double	9.969209968386869E36
attribute	thymidine	actual_range	double	-999.0, 8.08
attribute	thymidine	column_name	String	col_594
attribute	thymidine	comment	String	incorporation of nutrients
attribute	thymidine	ioos_category	String	Unknown
attribute	thymidine	long_name	String	Thymidine Incorp.
attribute	thymidine	lter_mscale	String	ratio
attribute	thymidine	lter_num_type	String	real
attribute	thymidine	lter_units	String	pmol/L/hr
attribute	thymidine	lter_units_string	String	picomolePerLiterPerHour
attribute	thymidine	units	String	picomol L-1 hr-1
attribute	thymidine	units_conventions	String	PAL-LTER
variable	leucine		double
attribute	leucine	_FillValue	double	9.969209968386869E36
attribute	leucine	actual_range	double	-999.0, 858.48
attribute	leucine	column_name	String	col_595
attribute	leucine	comment	String	incorporation of nutrients
attribute	leucine	ioos_category	String	Unknown
attribute	leucine	long_name	String	Leucine Incorp.
attribute	leucine	lter_mscale	String	ratio
attribute	leucine	lter_num_type	String	real
attribute	leucine	lter_units	String	pmol/L/hr
attribute	leucine	lter_units_string	String	picomolePerLiterPerHour
attribute	leucine	units	String	picomol L-1 hr-1
attribute	leucine	units_conventions	String	PAL-LTER
variable	hna_cell_concentration		double
attribute	hna_cell_concentration	_FillValue	double	9.969209968386869E36
attribute	hna_cell_concentration	actual_range	double	0.0, 2.9E9
attribute	hna_cell_concentration	colorBarMaximum	double	100.0
attribute	hna_cell_concentration	colorBarMinimum	double	0.0
attribute	hna_cell_concentration	column_name	String	col_5313
attribute	hna_cell_concentration	comment	String	High nucleic acid containing bacterial cells
attribute	hna_cell_concentration	ioos_category	String	Statistics
attribute	hna_cell_concentration	long_name	String	HNA
attribute	hna_cell_concentration	lter_mscale	String	ratio
attribute	hna_cell_concentration	lter_num_type	String	integer
attribute	hna_cell_concentration	lter_units	String	num/L
attribute	hna_cell_concentration	lter_units_string	String	numberPerLiter
attribute	hna_cell_concentration	units	String	count L-1
attribute	hna_cell_concentration	units_conventions	String	EML-2.0.1,EML-2.1.0,EML-LTER,NTL-LTER
variable	lna_cell_concentration		double
attribute	lna_cell_concentration	_FillValue	double	9.969209968386869E36
attribute	lna_cell_concentration	actual_range	double	0.0, 6.44384615E8
attribute	lna_cell_concentration	colorBarMaximum	double	100.0
attribute	lna_cell_concentration	colorBarMinimum	double	0.0
attribute	lna_cell_concentration	column_name	String	col_5314
attribute	lna_cell_concentration	comment	String	Low nucleic acid containing bacterial cells
attribute	lna_cell_concentration	ioos_category	String	Statistics
attribute	lna_cell_concentration	long_name	String	LNA
attribute	lna_cell_concentration	lter_mscale	String	ratio
attribute	lna_cell_concentration	lter_num_type	String	integer
attribute	lna_cell_concentration	lter_units	String	num/L
attribute	lna_cell_concentration	lter_units_string	String	numberPerLiter
attribute	lna_cell_concentration	units	String	count L-1
attribute	lna_cell_concentration	units_conventions	String	EML-2.0.1,EML-2.1.0,EML-LTER,NTL-LTER
variable	notes		String
attribute	notes	column_name	String	col_3698
attribute	notes	comment	String	Sampling and analysis notes
attribute	notes	ioos_category	String	Unknown
attribute	notes	long_name	String	Notes
attribute	notes	lter_mscale	String	nominal

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.